We estimated pigments and painting techniques with nondestructive analysis for Yu Eonho’s portrait made in the eighteenth century, then compared with 11 portraits and painting characteristics at that time. The pigments used to Yu Eon-ho's portrait include lead white, yellow dye, cinnabar, minium, and pink dye, malachite, azurite, iron oxide red and brown dye, blue and pink dye for purple. In the result compared with painted pigments of 11 portraits, iron oxide red without cinnabar was used on the face part and organic green dye only was used instead of inorganic pigments on the other side of clothing after Yu Eonho’s portraits portrait. This study is show the painting techniques on the portraits in the late 18th century. We expect to use as useful referencing data for the study on the coloring technique of a portrait in the late Joseon Dynasty.