Large scale anoxic chamber system(volume 28m3) was developed and installed at The National Folk Museum of Korea for the first time in Korea. In order to get optimal anoxic treatment condition, we compared the disinfection of adults, larvae and eggs of cigarette beetles using nitrogen and argon. The time for complete disinfection of cigarette beetles in pine wooden blocks exposed to nitrogen at oxygen concentration 0.01% and 50% in relative humidity were 15 days at 20℃, 10 days at 25℃, and 7 days 30℃. Time were 10 days at 20℃, 7 days at 25℃, and 5 days 30℃ in argon anoxic atmosphere. From the mortality of cigarette beetles, optimal disinfection condition was oxygen concentration 0.01%, 25℃ in temperature, 50% in relative humidity and exposure time 21 days at nitrogen atmosphere. And when large scale anoxic chamber system was supplied nitrogen by nitrogen generator for anoxic treatment of many collections or large collections, it could be operated stably. To verify optimal disinfection condition, museum insects(adults, larvae, pupae and eggs of cigarette beetles in pine wooden blocks, cotton fabrics and Korean paper book, adults and larvae of drugstore beetles in pine wooden blocks, cotton fabrics and Korean paper book, larvae of varied carpet beetles in pine wooden block and silk fabrics, adults and larvae of hide beetles and adults of rice weevils in breeding boxes) which exposed at optimal disinfection condition, were completely killed.