Anoxia Treatment using inert gas like nitrogen and argon has been used to eradicate insects successfully in museums as alternative of methyl bromide and toxic insecticide. Killing efficacy of insect for anoxia treatment is depend on species of insects, oxygen concentration, temperature, relative humidity and gas. It is possible to kill museum insects which are most tolerant in anoxia environment, within 1 month below 0.03% of oxygen concentration in temperature 15~25℃ and relative humidity 40~60% of museum environment. And various systems like bag, tent, bubble and chamber depending on size and quantity of objects, are used.