In this study, there has been investigated the simulation of irradiation dose using Monte Carlo methodology and experimental substantiation for the biological control of wooden cultural property. In the evaluation of fungal contamination on wooden cultural property, Dongyae, from exhibition storage, Aureobasidium pullulans was mainly identified. But these microorganisms were completely inactivated by 20 kGy gamma irradiation. For dosimetry simulation of Dongyae, Monte Carlo methodology with MCNP was used. The real dosimetry was measured using alanin dosimeters (at 7 different points on the front plan and 7 points on the back plan). Simulated and experimental results are compared and good agreement is observed. These result shows that irradiation can offer biologic control of wooden cultural property by optimal irradiation dose through high penetration power and Monte Carlo simulation.