To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, methanol extracts from 11 wood species which populate in Korea were screened for antifungal and insecticidal activity against 9 wood contaminant fungi and adult of Lasioderma serricorne. Antifungal activity of extracts was tested by using paper disc soaking method and feeder strip test against wood-rot fungi and wood surface contaminant fungi. Among these extracts, the most significant antimicrobial activity was observed from the extract of Phellodendron amurense at 5 ㎎/disc against Coniophora puteana, Lentinus lepideus, Tyromyces palustris and Aspergillus niger. The insecticidal activity of extracts was examined by topical application against L. serricorne adults. The extracts from Taxus cuspidata and Abies holophylla gave 56.7 % mortality at 25 ㎍/adult for 24 hr. From these result, the methanol extract from T. cuspidata, A. holophylla and P. amurense described could be useful for conservation of organic cultural heritage against biological deterioration by insect and wood contaminant fungi.